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- In relation to this article, we declare that there is no conflict of interest.
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Received September 18, 2015
Accepted November 10, 2015
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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식물세포배양으로부터 파클리탁셀 회수를 위한 초음파를 이용한 액-액 추출
Ultrasound-Assisted Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures
공주대학교 화학공학부, 31080 충남 천안시 서북구 천안대로 1223-24
Department of Chemical Engineering, Kongju National University, 1223-24, Cheonan-daero, Seobuk-gu, Cheonan, Chungnam, 31080, Korea
Korean Chemical Engineering Research, April 2016, 54(2), 229-233(5), 10.9713/kcer.2016.54.2.229 Epub 5 April 2016
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Abstract
본 연구에서는 식물세포배양액으로부터 파클리탁셀을 효율적으로 회수하기 위하여 초음파를 이용한 액-액 추출 공정을 개발하였다. 액-액 추출을 위한 최적의 초음파 파워와 조업 시간은 주어진 하층(메틸렌 클로라이드 층)/상층(메탄올 농축액 층) 비(25%, v/v)에서 각각 250W와 15 min임을 알 수 있었다. 최적 조건 하에서 초음파를 이용한 액-액 추출 공정의 경우 단 1 회 추출로 대부분의 파클리탁셀을 하층(메틸렌 클로라이드 층)으로부터 회수(~92%) 가능하였다. 또한 무기염 첨가에 의한 초음파 상승효과로 인하여 액-액 추출을 위하여 적절한 무기염 농도와 초음파 파워가 요구됨을 알 수 있었다.
In this study, an efficient ultrasound-assisted liquid-liquid extraction process was developed for recovering of paclitaxel from plant cell cultures. The optimal ultrasonic power and operating time were 250 W and 15 min at fixed ratio of bottom phase, methylene chloride to top phase, MeOH (25%, v/v). Under the optimal conditions developed in the present method, most of the paclitaxel (~92%) was recovered from crude extract by a single extraction step. Due to the synergistic effect of ultrasound by the addition of inorganic salt, an appropriate inorganic salt concentration and the ultrasonic power were found to be required for the effective recovery of paclitaxel using ultrasound-assisted liquid-liquid extraction.
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Ozuna C, Puig A, Garcia-Perez JV, Mulet A, Carcel JA, J. Food Eng., 119(1), 84 (2013)
