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Language: korean

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received March 3, 2016
Accepted March 31, 2016

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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에탄올 유기용매 전처리를 이용한 옥수수대의 효소당화

The Effect of Enzymatic Hydrolysis by Ethanol Organosolv Pretreatment of Corn Stover

박장한 김태현¹ 김준석^†

Jang Han Park Tae Huyn Kim¹ Jun Seok Kim^†

경기대학교 화학공학과, 16227 경기도 수원시 영통구 광교산로 154-42 ¹공주대학교 환경공학과, 31080 충남 천안시 서북구 천안대로 1223-24

Department of Chemical Engineering, Kyonggi University, 154-42, Gwanggyosan-ro, Yeongtong-gu, Suwon, Gyeonggi, 16227, Korea ¹Department of Environmental Engineering, Kongju National University, 1223-24, Cheonan-daero, Seobuk-gu, Cheonan, Chungnam, 31080, Korea

Korean Chemical Engineering Research, August 2016, 54(4), 448-452(5), 10.9713/kcer.2016.54.4.448 Epub 2 August 2016

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Abstract

새로운 수송용 에너지자원으로 각광받는 바이오매스의 효율적인 당화를 위한 전처리방법이 연구되고 있다. 최근 바이오매스의 에너지 전환 공정 중 전처리 비용이 높은 비중을 차지하고 있으며 이중 폐수처리가 커다란 문제점으로 지적되고 있다. 따라서 폐수발생을 줄이고 재사용이 용이한 유기용매(Organosolv)를 이용한 전처리를 수행하였으며, 전처리 바이오매스의 잔류 고형물의 양과 제거된 성분의 양을 이용하여 바이오매스의 전처리 효과를 효소당화를 통해 알아보았다. 전처리에 사용한 유기용매로는 99.5 wt% 에탄올을 사용하였고, 초본계 바이오매스인 옥수수대(corn stover)를 이용하여 전처리 하였다. 전처리 효과는 130~190 ℃ 조건에서 시간대별로 진행하여 전처리된 바이오매스의 효소당화를 통하여 확인하였다. 효소당화결과로 가장 높은 글루코오스 당화율을 보였던 전처리 온도는 190 ℃에서 반응시간 70분 이상의 조건 이였으며, 이 때 68% 이상의 당화율을 얻을 수 있었다. 또한 전처리 바이오매스의 잔류 고형물(Solid remaining)은 70% 이상이었고, 대부분의 셀룰로오스(Cellulose)와 헤미셀룰로오스(Hemicellulose)의 손실이 미비하여 대부분의 당 성분을 회수할 수 있다는 장점을 보였다.

This study is for the effective pretreatment and saccharification of lignocellulosic biomass for a transport fuel receiving attention. The waste water during the pretreatment of biomass is major factor for determining the price of biofuel. Therefore, we conducted high concentration of organosolv pretreatment for decline waste water and reusing the solvent. We confirmed effect of organosolv pretreatment by components analysis and enzymatic hydrolysis of pretreated biomass. The corn stover was used for and 99.5 wt% of ethanol as a organosolv pretreatment. The pretreatment condition was varied 130 to 190 ℃ during the designated reaction times and the effect of pretreatment was investigated by enzymatic hydrolysis. The highest glucose conversion was more than 68% the pretreatment condition of 190 ℃ for 70 min or more. The solid remaining was more than 70% and almost of cellulose and hemicellulose were survived.

Keywords

Organosolv pretreatment Enzyme hydrolysis Corn stover Solid remaining

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