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Received August 9, 2016
Accepted September 6, 2016
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황벽나무, 두충나무 추출물 등을 포함한 천연 추출물의 항균력 및 안전성
Antimicrobial Activity and Safety Test of Natural extract including Phellodendro namurense, Eucommia ulmides Oliv extracts
인제대학교 제약공학과, 50834 경남 김해시 어방동 607 1바이오메틱스, 46706 부산광역시 강서구 강동동 94-11
Department of Pharmaceutical Engineering, Inje University, 607, Eobang-dong, Gimhae, Gyongnam, 50834, Korea 1Biometics, 94-11, Gangdong-dong, Gangseo-gu, Busan, 46706, Korea
pedkim@inje.ac.kr
Korean Chemical Engineering Research, December 2016, 54(6), 762-766(5), 10.9713/kcer.2016.54.6.762 Epub 5 December 2016
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Abstract
본 연구에서는 황벽나무, 두충나무, 귀룽나무 추출물 등을 포함하는 천연추출물에대해 항균활성 효과 및 안전성에 대해 검토하였다. 항균활성은 기회 병원성균인 Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli 및 Candida albicans에 대해 실험되었다. 안전성 시험으로는 세포독성실험, 단회 경구투여 독성시험, 단회 흡입투여 독성시험, 반복 흡입투여 독성시험, 안점막 자극시험이 실시되었다. 항균력효과시험 결과 추출물은 Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans 4가지 균 모두에 대해 우수한 항균활성을 보여주었다. 천연추출물의 단회 경구투여 독성시험, 단회 흡입투여 독성시험 및 반복 흡입투여 독성시험, 안점막 자극시험의 결과 독성은 관찰되지 않았다. 따라서 황벽나무, 두충나무, 귀룽나무 추출물 등을 포함한 천연추출물은 뛰어난 안전성과 항균력이 있음을 확인하였다.
In this study, natural extract including Phellodendro namurense, Eucommia ulmides Oliv and Prunus padus were tested for antimicrobial activity and safety. Antimicrobial activity was measured by using opportunistic microbes such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans. As safety test, cell viability test, single dose oral toxicity test, single dose inhalation toxicity test, repeat dose inhalation toxicity test and eye irritation test were done. From antimicrobial test, natural extract showed execellent antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Escherichia coli. From 5 kinds of safety tests, toxicity was not observed. From experimental results, natural extract including Phellodendro namurense, Eucommia ulmides Oliv and Prunus padus showed superb safety and antimicrobial effect.
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Park JC, Functional Food and oriental Medicine, Hyoilbooks, Seoul(2007).
Association of Official Analytical Chemists, A.O.A.C., Official methods of analysis, 15thed., Washington DC. (1990).
Jung DH, Yoon BH, Lee YH, Tea Living Culture Book (2012).
Mosmann T, J. Immunol. Methods, 65, 55 (1983)
Kim H, Shin H, Hwang D, Lee J, Bak M, Kim J, Kim D, Korean Chem. Eng. Res., 51(5), 536 (2013)
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Lowy FD, N. Engl. J. Med., 339, 520 (1998)
El Solh AA, Alhajhusain A, J. Antimicrob. Chemoter., 64, 229 (2009)
