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Received March 27, 2017
Accepted April 10, 2017
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Fabrication of Hemoglobin/Silver Nanoparticle Heterolayer for Electrochemical Signal-enhanced Bioelectronic Application
Department of Chemical Engineering, Kwangwoon University, 20, Kwangwoon-ro, Nowon-gu, Seoul, 01897, Korea 1Department of Chemical & Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul, 04107, Korea
jwchoi@sogang.ac.kr
Korean Chemical Engineering Research, August 2017, 55(4), 556-560(5), 10.9713/kcer.2017.55.4.556 Epub 4 August 2017
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Abstract
A hemoglobin/silver nanoparticle heterolayer was fabricated for bioelectronic device with electrochemical signal-enhancement effect. As a device element, a hemoglobin, the metalloprotein, contained the heme group that showed the redox property was introduced for charge storage element. For electron transfer facilitation, a silver nanoparticle was introduced for electrochemical signal facilitation, the hemoglobin was immobilized onto Au substrate using chemical linker 6-mercaptohexanoic acid (6-MHA). Then, the silver nanoparticle was immobilized onto fabricated hemoglobin/6-MHA heterolayers by layer-by-layer (LbL) method. The surface morphology and surface roughness of fabricated heterolayer were investigated by atomic force microscopy (AFM). The redox property of hemoglobin/silver nanoparticle heterolayer was investigated by a cyclic voltammetry (CV) experiment for obtaining an oxidation potential and reduction potential. Moreover, for the assessing charge storage function, a chronoamperometry (CA) experiment was conducted to hemoglobin/silver nanoparticle-modified heterolayer electrode using oxidation and reduction potentials, respectively. Based on the results, the fabricated hemoglobin/silver nanoparticle heterolayer showed that an increased charge storage effect compared to hemoglobin monolayer-modified electrode.
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References
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