본 연구에서는 Poly (dimethylsiloxane) (PDMS)의 높은 소수성과 생체분자들의 비특이적 흡착 문제를 해결하기 위해 PEO-PPO-PEO 블록 공중합체의 포매(embeddeing) 방식을 이용하여 손쉬운 표면 개질 및 이의 최적화 조건을 조사 하였다. 친수성 표면 개질의 특성은 PDMS 내에 포매된 블록 공중합체의 농도, 수침(water-soaking), 및 소수성 표면으로 회복 시간 등의 영향을 평가하였다. 개질된 PDMS 표면은 알부민 단백질(2 mg/ml)까지 단백질의 비특이적 결합 방지 특성을 보였으며, 또한 O/W (Oil-in-Water) 에멀젼을 쉽게 형성할 수 있었다.

In this study, we optimized a method of PEO-PPO-PEO block copolymer embedding, for solving non-specific protein and biomolecular adsorption and high hydrophobicic surface property, which is widely known as problems of poly (dimethylsiloxane) (PDMS) that has frequently been used in basic biological and its applied research. We assessed its surface modification by controlling the concentration of embedded block copolymer, water-soaking time, and recovery time as variables by contact angle measurements. In order to evaluate its antifouling ability, adsorption of FITC-BSA molecules was quantified. Furthermore, we generated oil-in-water (O/W) emulsion as a proof-of-concept experiment to confirm that the optimized surface modification works properly.

Surface modification Antifouling Microfluidics Triblock copolymer PDMS Oil-in-water emulsion

Hillborg H, Gedde UW, Ieee T. Dielect. El. In., 6(5), 703 (1999)
McDonald JC, Whitesides GM, Accounts Chem. Res., 35(7), 491 (2002)
Sia SK, Whitesides GM, Electroanalysis, 24, 3563 (2003)
Lee D, Kim YT, Lee JW, Kim DH, Seo TS, Korean J. Chem. Eng., 33(9), 2644 (2016)
Singh R, Lee HJ, Singh AK, Kim DP, Korean J. Chem. Eng., 33(8), 2253 (2016)
Lee Y, Jung I, Na J, Park S, Kshetrimayum KS, Han C, Korean Chem. Eng. Res., 53(6), 818 (2015)
Dahlin AP, Bergstrom SK, Andren PE, Markides KE, Bergquist J, Anal. Chem., 77, 5356 (2005)
Jeong SG, Kim J, Jin SH, Park KS, Lee CS, Korean J. Chem. Eng., 33(10), 2761 (2016)
Sung YJ, Kwak HS, Choi HI, Kim JYH, Sim SJ, Korean Chem. Eng. Res., 55(1), 80 (2017)
Lee S, Jeong W, Beebe DJ, Lab Chip, 3(3), 164 (2003)
Unger MA, Chou HP, Thorsen T, Scherer A, Quake SR, Science, 288(5463), 113 (2000)
Faid K, Voicu R, Bani-Yaghoub M, Tremblay R, Mealing G, Py C, Barjovanu R, Biomed. Microdevices, 7(3), 179 (2005)
Russell MT, Pingree LSC, Hersam MC, Marks TJ, Langmuir, 22(15), 6712 (2006)
Kim GY, Jeong HH, Lee CS, Roh C, Korean Chem. Eng. Res., 54(2), 223 (2016)
Baret JC, Miller OJ, Taly V, Ryckelynck M, El-Harrak A, Frenz L, Rick C, Samuels ML, Hutchison JB, Agresti JJ, Link DR, Weitz DA, Griffiths AD, Lab Chip, 9(11), 1850 (2009)
Cho SH, Chen CH, Tsai FS, Godin JM, Lo YH, Lab Chip, 10(12), 1567 (2010)
Fu AY, Spence C, Scherer A, Arnold FH, Quake SR, Nat. Biotechnol., 17, 1109 (1999)
Karir T, Hassan PA, Kulshreshtha SK, Samuel G, Sivaprasad N, Meera V, Anal. Chem., 78(11), 3577 (2006)
Toepke MW, Beebe DJ, Lab Chip, 6(12), 1484 (2006)
Kim P, Lee SE, Jung HS, Lee HY, Kawai T, Suh KY, Lab Chip, 6(1), 54 (2006)
Lee S, Voros J, Langmuir, 21(25), 11957 (2005)
Kovach KM, Capadona JR, Sen Gupta A, Potkay JA, J. Biomed. Mater. Res., 102(12), 4195 (2014)
Zhang HB, Chiao M, J. Med. Biol. Eng., 35(2), 143 (2015)
Xu JJ, Gleason KK, Chem. Mater., 22(5), 1732 (2010)
Yang R, Gleason KK, Langmuir, 28(33), 12266 (2012)
Huang B, Wu HK, Kim S, Kobilka BK, Zare RN, Lab Chip, 6(3), 369 (2006)
Wu ZG, Hjort K, Lab Chip, 9(11), 1500 (2009)
Zhou JW, Khodakov DA, Ellis AV, Voelcker NH, Electrophoresis, 33(1), 89 (2012)
Kim J, Chaudhury MK, Owen MJ, J. Colloid Interface Sci., 226(2), 231 (2000)
Hillborg H, Sandelin M, Gedde UW, Polymer, 42(17), 7349 (2001)
Fritz JL, Owen MJ, J. Adhes., 54(1-2), 33 (1995)
Kim J, Chaudhury MK, Owen MJ, Orbeck T, J. Colloid Interface Sci., 244(1), 200 (2001)
Dong BY, Manolache S, Wong ACL, Denes FS, Polym. Bull., 66(4), 517 (2011)