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Received April 10, 2018
Accepted May 8, 2018
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미세조류로부터의 에너지 효율적인 Astaxanthin 회수 기술 개발
Recovery of Astaxanthin from microalgae Using Simple and Energy-efficient Method
한남대학교 화공신소재공학과, 34054 대전광역시 유성구 유성대로 1646 1부산대학교 화공생명공학부, 46241 부산광역시 금정구 부산대학로 63번길 2
Department of Advanced Materials & Chemical Engineering, Hannam University, 1646, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Korea 1Department of Chemical & Biomolecular Engineering, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan, 46241, Korea
Korean Chemical Engineering Research, June 2018, 56(3), 376-380(5), 10.9713/kcer.2018.56.3.376 Epub 4 June 2018
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Abstract
강력한 항산화물질인 astaxanthin의 함량이 다른 천연 공급원에 비해 높아 astaxanthin 생산균주로 주목받고 있는 Haematococcus pluvialis는 상당한 두께의 견고한 세포벽을 가지고 있어, 세포 파쇄를 위해 많은 에너지가 소모되고 비용이 비싼 방법들이 이용되고 있다. 이에 H. pluvialis로부터 막자와 막자사발을 이용하여 astaxanthin을 손쉽게 효율적으로 추출하는 방법을 제시하였다. 막자와 막자사발을 이용하여 분쇄한 후 추출용매로 acetonitrile, acetone, methanol, dichloromethane : methanol (1:3, v/v), ethylacetate : ethanol (1:1, v/v)로 사용하여 비교하였을 때, acetone을 이용하였을 때 astaxanthin을 1.13~1.29 배 더 높은 효율로 추출할 수 있었다. 또한 acetone으로 H. pluvialis로부터 추출할 경우, 1차 추출로 H. pluvialis에 축적되어 있는 전체 astaxanthin의 96.7%를 회수할 수 있을 정도로 acetone은 astaxanthin 추출효율이 높았다. H. pluvialis가 세포내에 축적하는 astaxanthin은 축적 특성상 ester-형태의 astaxanthin로 다량 축적하므로, 추출물 내의 다양한 형태의 astaxanthin을 분리하기 위하여 농도 구배 시스템을 적용한 HPLC 분석을 수행하였다. H. pluvialis에 축적되어 있는 전체 astaxanthin 중 free astaxanthin이 45.9%이고, 나머지 54.1%는 ester-형태의 astaxanthin이었다.
The astaxanthin recovery efficiencies were compared in acetonitrile, acetone, methanol, dichloromethane : methanol (1:3, v/v) and ethylacetate : ethanol (1:1, v/v) as a extraction solvent after the grinding of the H. pluvialis cells. The astaxanthin extraction yield in acetone was 1.13~1.29 times higher than other extraction solvents. It was also found that 96.7% of astaxanthin accumulated in H. pluvialis could be recovered by a single extraction. Since astaxanthin exists mainly as astaxanthin esters in H. pluvialis, a gradient reversed-phase HPLC analysis was carried out for the separation of astaxanthin esters from the extracts of H. pluvialis. Among the astaxanthin inside the H. pluvialis cell, free astaxanthin was 45.9% and astaxanthin esters were the rest.
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