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Received September 14, 2018
Accepted October 4, 2018
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미세 성형 방법을 이용한 형광 표지된 이중 분획 입자의 제조
Fabrication of Fluorescent Labeled Bi-compartmental Particles via the Micromolding Method
충남대학교 응용화학공학과, 34134 대전광역시 유성구 대학로 99
Department of Chemical Engineering and Applied Chemistry, Chungnam National University, 99, Daehak-ro, Yuseong-gu, Deajeon, 34134, Korea
rhadum@cnu.ac.kr
Korean Chemical Engineering Research, December 2018, 56(6), 826-831(6), 10.9713/kcer.2018.56.6.826 Epub 4 December 2018
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Abstract
본 연구는 다중 형광이 표지된 이중 분획 입자의 제조에 관한 것이다. 입자 내에서 형광 발현을 분획화하기 위하여, 형광의 여기 및 방출 스펙트럼의 중첩이 적은 두가지의 형광 염료를 선정한다. 또한, 형광 안정성을 확보하기 위하여 선정된 형광 염료는 입자를 구성하는 소재와 함께 가교될 수 있도록 분자 내에 아크릴레이트(acrylate) 작용기를 포함한다. 공초점 현미경 촬영을 통하여 선정된 형광 물질을 이용하여 제조된 입자에서 강한 형광 발현 및 형광의 분획화를 확인하였다. 더 나아가 4주 동안 형광 발현 및 세기를 측정하여 장기간의 형광 안정성을 검증하였다. 본 연구에서 제조된 다중 형광 표지된 이중 분획 입자는 다중 표적형 약물 전달 체계, 3차원 브라운 운동의 해석 연구, 3차원의 복잡한 자기 조립체 형상의 규명 연구 등에 널리 활용될 수 있으리라 기대한다.
This study presents fabrication of bi-compartmental particles labeled by multiple fluorescence. To compartmentalize fluorescent expression at the particle, two fluorescent dyes with less overlap of the excitation and emission spectra are selected. To ensure the fluorescence stability, the fluorescent dyes contain acrylate functional groups in the molecules so that they can be cross-linked together with monomers constituting the particle. Strong fluorescent expression and compartmentalization were observed at the particle fabricated using the selected fluorescent dyes through confocal microscopy. Furthermore, long-term fluorescence stability was verified by measuring fluorescent expression and intensity for 4 weeks. We anticipate that the bi-compartmental particles labeled by multiple fluorescence can be widely used for multi-target drug delivery system, analysis of 3 dimensional Brownian motion, and investigation of 3 dimensional complex self-assembled morphologies.
Keywords
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Blaaderen AV, Peetermans J, Maret G, Dhont JKG, J. Chem. Phys., 96(6), 4591 (1992)
Moschakis T, Murray BS, Dickinson E, Langmuir, 22(10), 4710 (2006)
Ruthardt N, Lamb DC, Brauchle C, Mol. Ther., 19(7), 1199 (2011)
Kim J, Choi CH, Yeom SJ, Eom N, Kang KK, Lee CS, Langmuir, 33(30), 7503 (2017)
Mohraz A, Solomon MJ, Langmuir, 21(12), 5298 (2005)
McGorty R, Fung J, Kaz D, Manoharan VN, Mater. Today, 13, 34 (2010)
Oh S, Kang WK, Kang JW, Kim KS, Lee H, Korean Chem. Eng. Res., 50(6), 1082 (2012)
Costanzo M, Carton F, Marengo A, Berlier G, Stella B, Arpicco S, Malatesta M, Eur. J. Histochem., 60(2), 107 (2016)
Zhang LW, Monteiro-Riviere NA, J. Biomed. Opt., 18(6), 061214 (2013)
Jeon W, Kim GY, Kim GH, Ha CS, Korean Chem. Eng. Res., 48(2), 178 (210)
Lee YC, Kang IJ, Korean Chem. Eng. Res., 54(2), 200 (2016)
Hwang J, Lee K, Gilad AA, Choi J, Biotechnol. Bioprocess Eng., 23, 144 (2018)
Reenan AV, Jong AMD, Toonder JMJD, Prins MWJ, Lab Chip, 14, 1966 (2014)
Bally M, Graule M, Parra F, Larson G, Hook F, Bioinerphases, 8(1), 1 (2013)
Bhunia SK, Saha A, Maity AR, Ray SC, Jana NR, Sci. Rep., 3(1473), 1 (2013)
Zrazhevskiy P, Sena M, Gao X, Chem. Soc. Rev., 39(11), 4326 (2010)
Chaudhary V, Bhowmick AK, Korean J. Chem. Eng., 32(8), 1707 (2015)
Lee EJ, Korean J. Chem. Eng., 35(9), 1765 (2018)
Kim YM, Kim JH, Park SC, Park YH, Kang MK, KSBB J., 31(4), 300 (2016)
Choi ES, Kang YY, Mok H, Biotechnol. Bioprocess Eng., 23, 150 (2018)
Lee JS, Go NK, Lee SY, Hur W, KSBB J., 29(5), 328 (2014)
Yi Y, Sanchez L, Gao Y, Yu Y, Analyst, 141(12), 3526 (2016)
Choi CH, Kang SM, Jin SH, Yi H, Lee CS, Langmuir, 31(4), 1328 (2015)
Hwang S, Lahann J, Macromol. Rapid Commun., 33(14), 1178 (2012)
Sanchez L, Patton P, Anthony SM, Yi Y, Yu Y, Soft Matter, 11, 5346 (2015)
Hong L, Cacciuto A, Luijten E, Granick S, Nano Lett., 6(11), 2510 (2006)
Kang SM, Choi CH, Kim J, Yeom SJ, Lee D, Park BJ, Lee CS, Soft Matter, 12, 5847 (2016)
Tang JL, Schoenwald K, Potter D, White D, Sulchek T, Langmuir, 28(26), 10033 (2012)
Nie ZH, Li W, Seo M, Xu SQ, Kumacheva E, J. Am. Chem. Soc., 128(29), 9408 (2006)
Seiffert S, Weitz DA, Polymer, 51(25), 5883 (2010)
Choi CH, Lee J, Yoon K, Tripathi A, Stone HA, Weitz DA, Lee CS, Angew. Chem.-Int. Edit., 49, 7748 (2010)
Yeom SJ, Kang SM, Kim J, Nam JO, Eom N, Lee S, Lee CS, Polym. Korea, 40(3), 457 (2016)
Love JC, Wolfe DB, Jacobs HO, Whitesides GM, Langmuir, 17(19), 6005 (2001)
Hwang S, Choi CH, Lee CS, Macromol. Res., 20(4), 422 (2012)
Shim GR, Yeom SJ, Jeong SG, Kang KK, Lee CS, Clean Technol., 24(2), 105 (2018)
Doytcheva M, Dotcheva D, Stamenova R, Tsvetanov C, Macromol. Mater. Eng., 286, 30 (2001)
Azzam WR, J. Appl. Polym. Sci., 123(1), 299 (2012)
https://www.thermofisher.com/kr/ko/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html.
