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Received October 13, 2020
Accepted November 11, 2020
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다이타이로신 공유결합으로 자기조립된 펩타이드 나노입자의 합성
Synthesis of Self-Assembled Peptide Nanoparticles Based on Dityrosine Covalent Bonds
경북대학교 의생명융합공학과, 41566 대구광역시 북구 대학로80
Biomedical Convergence Science and Technology, Kyungpook National University, 80 Daehakro, Bukgu, Daegu, 41566, Korea
kimin@knu.ac.kr
Korean Chemical Engineering Research, February 2021, 59(1), 112-117(6), 10.9713/kcer.2021.59.1.112 Epub 15 January 2021
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Abstract
본 연구에서는 생물학적 공유결합인 다이타이로신 결합을 모방하여 비가역적 공유결합을 기반으로 한 펩타이드의 자기조립 방법을 연구하였다. 고밀도의 다이타이로신 결합을 달성하기 위해 Tyr-Tyr-Leu-Tyr-Tyr (YYLYY) 의 서열을 갖는 펩타이드 단량체를 선택하였다. 다이타이로신 결합으로 자기조립 된 펩타이드 나노입자는 가시광선 하에서 Ru(BPY)3Cl2 촉매를 사용하여 단일공정 광가교를 통해 합성되었다. 펩타이드 나노 입자의 크기에 대한 각 성분의 농도 효과는 동적 광산란, UV-Vis 분광법 및 투과 전자 현미경을 사용하여 확인하였다. 이를 통해 130 nm~350 nm범위의 펩타이드 나노입자의 크기별 최적의 합성 조건을 제시하였다.
In this study, a method of self-assembly of peptides based on irreversible covalent bonds was studied by mimicking a biological covalent bond, dityrosine bond. A tyrosine-rich short peptide monomer having the sequence of Tyr-Tyr-Leu-Tyr-Tyr (YYLYY) was selected to achieve a high-density of dityrosine bond. The peptide nanoparticles covalently self-assembled with dityrosine bonds were synthesized by one-step photo-crosslinking of a peptide using a ruthenium catalyst under visible light. The effect of the concentration of each component for the size of the peptide nanoparticle was studied using dynamic light scattering, UV-Vis spectroscopy, and transmission electron microscopy. As a result, the synthesis conditions for size of the peptide nanoparticles ranging from 130 nm to 350 nm were optimized.
Keywords
References
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