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Received December 14, 2021
Accepted February 9, 2022
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Improved Ectoine Production from Methane by Optimization of the Bio-milking Process in Engineered Methylomicrobium alcaliphilum 20Z
1C1 Gas Refinery R&D Center, Sogang University, Seoul, 04107, Korea 2Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 04107, Korea
jinwonlee@sogang.ac.kr
Korean Chemical Engineering Research, August 2022, 60(3), 392-397(6), 10.9713/kcer.2022.60.3.392 Epub 18 July 2022
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Abstract
Methane is one of the major greenhouse gases, recently, the biotechnological conversion from methane to high-value added chemicals have emerged as an effort to reduce methane gas emission. In this study, we optimized ectoine bio-milking conditions in which cells were repeatedly used to improve intracellular and extracellular ectoine yield from methane by using Methylomicrobium alcaliphilum 20ZDP2. First, the cultivation and intracellular ectoine accumulation conditions were optimized with respect to the growth phase and medium salinity to achieve the highest yield of synthesis. Second, ectoine excretion was optimized by determining the ectoine secretion time (15 min) in appropriate medium salinity under hypoosmotic conditions (1% NaCl). Finally, bio-milking of ectoine was successfully repeated more than 10 times using M. alcaliphilum 20ZDP2, and the ectoine yield was improved up to 129.29 mg/ DCW g.
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References
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Galinski EA, Pfeiffer HP, Trüper HG, Eur. J. Biochem., 149(1), 135 (1985)
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Jebbar M, Talibart R, Gloux K, Bernard T, Blanco C, J. Bacteriol., 174(15), 5027 (1992)
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Kunte HJ, Galinski EA, Trüper HG, J. Microbiol. Methods, 17, 129 (1993)
Semrau JD, DiSpirito AA, Yoon S, Fems Microbiol. Rev., 34(4), 496 (2010)
Chu F, Lidstrom ME, J. Bacteriol., 00959 (2016)
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Cantera S, Lebrero R, Sadornil L, García-Encina PA, Muñoz R, J. Environ. Manage., 182, 160 (2016)
Cantera S, Lebrero R, Rodríguez S, García-Encina PA, Muñoz R, Chem. Eng. J., 328, 44 (2017)
Cantera S, Lebrero R, Rodríguez E, García-Encina PA, Muñoz R, J. Clean Prod., 152, 134 (2017)
Cantera S, Phandanouvong-Lazano V, Pascual C, García-Encina PA, Lebrero R, Hay A, Muñoz R, Waste Manage., 102, 773 (2020)
