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- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
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Received August 29, 2022
Revised November 10, 2022
Accepted November 15, 2022
- Acknowledgements
- The authors acknowledge the profound financial support from TNB Research Sdn. Bhd., Malaysia through Fundamental Research Grant Scheme PV035-2021.
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Experimental Assessment of Mesophilic and Thermophilic Batch Fermentative Biohydrogen Production from Palm Oil Mill Effluent Using Response Surface Methodology
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Abstract
The present work evaluated the production of biohydrogen under mesophilic and thermophilic conditions
through dark fermentation of palm oil mill effluent (POME) in batch mode using the design of experiment methodology.
Response surface methodology (RSM) was applied to investigate the influence of the two significant parameters, POME
concentration as substrate (5, 12.5, and 20 g/l), and volumetric substrate to inoculum ratio (1:1, 1:1.5, and 1:2, v/v.%),
with inoculum concentration of 14.3 g VSS/l. All the experiments were analyzed at 37 ℃ and 55 ℃ at an incubation
time of 24 h. The highest chemical oxygen demand (COD) removal, hydrogen content (H2%), and hydrogen yield (HY)
at a substrate concentration of 12.5 g COD/l and S:I ratio of 1:1.5 in mesophilic and thermophilic conditions were obtained
(27.3, 24.2%), (57.92, 66.24%), and (6.43, 12.27 ml H2/g CODrem), respectively. The results show that thermophilic
temperature in terms of COD removal was more effective for higher COD concentrations than for lower concentrations.
Optimum parameters projected by RSM with S:I ratio of 1:1.6 and POME concentration of 14.3 g COD/l showed higher
results in both temperatures. It is recognized how RSM and optimization processes can predict and affect the process
performance under different operational conditions.
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