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Received May 18, 2023
Revised June 2, 2023
Accepted June 5, 2023
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자성 미세입자에 고정된 효소를 통한 요소 전환

Urea Conversion via Enzymes Immobilized on Magnetic Microparticles

1서울과학기술대학교 화공생명공학과 01811 서울특별시 노원구 공릉로 232 2K-water연구원 K-water 34350 대전광역시 대덕구 신탄진로 200
1Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology, Gongreung-ro 232, Nowon-gu, Seoul, 01811, Korea 2K-water Research Institute Water & Wastewater Research Center, 125, Yuseong-daero 1689beon-gil, Yuseong-gu, Daejeon, 34045, Korea
jwpark@seoultech.ac.kr, kh.lee@kwater.or.kr
Korean Chemical Engineering Research, August 2023, 61(3), 407-411(5), 10.9713/kcer.2023.61.3.407 Epub 31 August 2023
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Abstract

요소분해효소가 자성입자표면에 공유결합으로 고정되어 요소의 중탄산음이온 전환을 촉진하였으며, 이 전환을 정성 적으로 고압액체크로마토그래피와 UV/Vis분광법으로 확인하고 순환전압전류법을 사용하여 정량적으로 분석하였다. 시 간에 따른 전환량을 측정하고 이를 반응속도식으로 분석하여 0.0474 min-1의 반응속도 상수를 산출하였다. 처음 1~3 회에서는 90% 이상의 전환율을 확인하였으며, 50% 전환율까지 8회 재활용이 가능하였다. 30일 동안의 저장에 대하여 평가된 안정성은 유지되는 것으로 관찰되었다. 본 연구의 결과, 지지체에 공유결합으로 고정된 요소분해효소는 초순수 제조 목적으로 요소제거에 활용될 수 있음을 알 수 있다.

The urease is covalently immobilized on the surface of the magnetic particles to catalyze the conversion of urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min-1. In the 1 to 3 cycles, a conversion percentage of over 90% was found, and it was possible to reuse the urease 8 times up to the percentage of 50%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the purpose of producing ultrapure water.

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