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- korean
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- In relation to this article, we declare that there is no conflict of interest.
- Publication history
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Received May 18, 2023
Revised June 2, 2023
Accepted June 5, 2023
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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자성 미세입자에 고정된 효소를 통한 요소 전환
Urea Conversion via Enzymes Immobilized on Magnetic Microparticles
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Abstract
요소분해효소가 자성입자표면에 공유결합으로 고정되어 요소의 중탄산음이온 전환을 촉진하였으며, 이 전환을 정성
적으로 고압액체크로마토그래피와 UV/Vis분광법으로 확인하고 순환전압전류법을 사용하여 정량적으로 분석하였다. 시
간에 따른 전환량을 측정하고 이를 반응속도식으로 분석하여 0.0474 min-1의 반응속도 상수를 산출하였다. 처음 1~3
회에서는 90% 이상의 전환율을 확인하였으며, 50% 전환율까지 8회 재활용이 가능하였다. 30일 동안의 저장에 대하여
평가된 안정성은 유지되는 것으로 관찰되었다. 본 연구의 결과, 지지체에 공유결합으로 고정된 요소분해효소는 초순수
제조 목적으로 요소제거에 활용될 수 있음을 알 수 있다.
The urease is covalently immobilized on the surface of the magnetic particles to catalyze the conversion of
urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and
UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to
time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min-1. In the
1 to 3 cycles, a conversion percentage of over 90% was found, and it was possible to reuse the urease 8 times up to the
percentage of 50%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result
of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the
purpose of producing ultrapure water.
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