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Received June 18, 2024
Revised July 7, 2024
Accepted July 7, 2024
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젖산 산화효소-카탈라아제-미토콘드리아 전극 제작 및 특성 분석

Fabrication and Characterization of Lactate Oxidase-catalase-mitochondria Electrode

경상국립대학교 화학공학과 및 그린에너지 연구소 1경상국립대학교 나노신소재융합공학과 2단국대학교 코스메디컬소재학과
Department of Chemical Engineering and RIGET 1Department of Materials Engineering and Convergence Technology, Gyeongsang National University 2Department of Cosmedical & Materials, College of Bio-convergence, Dankook University
cj_kim@gnu.ac.kr
Korean Chemical Engineering Research, August 2024, 62(3), 238-245(8), https://doi.org/10.9713/kcer.2024.62.3.238 Epub 1 August 2024
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Abstract

젖산 전극은 환자의 건강상태와 스트레스 수준, 및 운동선수의 피로도를 실시간으로 모니터링하는 젖산 센서 또는

젖산 연료전지 전극으로 활용될 수 있다. 본 연구에서는 젖산 산화효소, 카탈라아제, 미토콘드리아로 구성된 고성능 전

극을 제작하고 전극의 표면분석 및 전기화학적 특성을 조사하였다. 단일벽 탄소나노튜브로 개질된 탄소종이(CPSWCNT)

는 개질 전보다 전기 전도성이 크게 향상되었다. 젖산 산화효소, 카탈라아제, 그리고 미토콘드리아가 부착된

전극(CP-SWCNT-LOx-Cat-Mito)은 젖산 산화효소와 카탈라아제가 부착된 전극에 비하여 많은 전류를 생산하였다. 빌

리루빈 산화효소(BOD)가 부착된 전극(CP-SWCNT-BOD)이 생산하는 환원전류량은 전해질의 산소 존재 유무에 따라

크게 영향을 받았다. CP-SWCNT-LOx-Cat-Mito (anode)와 CP-SWCNT-BOD (cathode)로 구성된 연료전지는 133 μA/

cm2로 방전 시 0.2 V의 셀 전위를 유지하며 29 μW/cm2의 전력을 생산하였다. 본 연구결과는 미토콘드리아가 젖산 센

서 및 연료전지 성능 향상에 필수적인 생체물질임을 시사한다.

The lactate electrode can be utilized either as an electrode for lactate sensor to monitor the patient's health

status, stress level, and athlete's fatigue in real time or lactate fuel cell. In this study, we fabricated a high-performance

electrode composed of lactate oxidase, catalase, and mitochondria, and investigated the surface analysis and electrochemical

properties of this electrode. Carbon paper modified with single-walled carbon nanotubes (CP-SWCNT) had significantly

improved electrical conductivity compared to before modification. The electrode to which lactate oxidase, catalase, and

mitochondria were attached (CP-SWCNT-LOx-Cat-Mito) produced a higher current than the electrode to which lactate

oxidase and catalase were attached. The amount of reduction current produced by the bilirubin oxidase (BOD)-attached

electrode (CP-SWCNT-BOD) was greatly affected by the presence or absence of oxygen in the electrolyte. The fuel cell

composed of CP-SWCNT-LOx-Cat-Mito (anode) and CP-SWCNT-BOD (cathode) produced maximum power (29 μW/

cm2) at a discharge current density of 133 μA/cm2. From this study, we had proved that mitochondria is essential for

improving lactate sensor and fuel cell performance.

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