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Received May 15, 2003
Accepted September 3, 2003
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Penicillium sp. ZD-Z1 Chitosanase Immobilized on DEAE Cellulose by Cross-linking Reaction
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China
Korean Journal of Chemical Engineering, January 2004, 21(1), 201-205(5), 10.1007/BF02705399
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Abstract
Chitosanase obtained from Penicillium sp.ZD-Z1 was immobilized on DEAE cellulose with glutaraldehyde by cross-linking reaction. The optimal conditions of immobilization were as follows: 0.1 g DEAE cellulose was treated with 5 ml 5% glutaraldehyde solution; then 2.3 mg chitosanase was immobilized on the carrier. The optimal temperature and pH was 60 ℃ and 4.0, and the Km value was 18.87 g/L. Under optimal conditions, the activity of immobilized enzyme is 1.5 U/g, and the recovery of enzyme activity is 81.3%. After immobilization, the optimal temperature and Km value increased (from 50 ℃ to 60 ℃, from 2.49 g/L to 18.87 g/L), whereas the optimal pH was reduced (from 5.0 to 4.0). The enzyme activity loss was less than 20% after 10 times batch reaction; the immobilized enzyme showed good operation stability.
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References
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Sugano M, Watanabe S, Kishi A, Izume M, Ohtakara A, Lipids, 23, 187 (1988)
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Suzuki K, Mikami T, Okawa Y, Tokoro A, Suzuki S, Suzuki M, Carbohyd. Res., 151, 403 (1986)
Suzuki S, Fragrance J., 15, 61 (1996)
Suzuki S, Watanabe T, Mikami T, Matsumoto T, Suzuki M, "Immuno-enhancing Effects of N-acetylchitohexaose," In: Brine, C.J., Sanford, P.A., Zikakis, J.P., Editors. Advances in Chitin and Chitosan. Barking, UK: Elsevier Applied Science, 96 (1992)
Tokoro A, Tatewaki N, Suzuki K, Mikami T, Suzuki S, Suzuki M, Chem. Pharm. Bull., 36, 784 (1988)
Tokoro A, Kobayashi M, Tatekawa N, Suzuki K, Okawa Y, Microbiol. Immunol., 33, 357 (1989)
Tsukada K, Matsumoto T, Aizawa K, Tokoro A, Naruse R, Suzuki S, Suzuki M, Jpn. J. Cancer. Res., 81, 259 (1990)
Uchida Y, Izume M, Ohtakara A, "Preparation of Chitosan Oligomers with Purified Chitosanase and its Application," In: SkjakBraek, G., Anthonsen, T. and Sandford, P., Editors. Chitin and Chitosan. Barking, UK: Elsevier Applied Science, 373 (1989)
Walker SM, Hadwiger L, Ryan CA, Biochem. Biophys. Res. Commun., 110, 194 (1983)
Yamada A, Shibbuya N, Kodama O, Akatsuka T, Biosci. Biotechnol. Biochem., 57, 405 (1993)
