Affinity Chromatography의 성질과 사용목적이 개략적으로 서술되었다. 또한 Chromatographic Column에 대한 수학적 모델을 세우고, Laplace변환에 의하여 구해진 moments를 이용하여 Breakthrough curve의 점근해를 구하는 방법이 설명되었다. Affinity Chromatography는 pH의 영향을 받는데 이를 설명하기 위하여 흡착현상의 모델을 설정하고 수학적 모델과의 상관관계를 설명하였다.

Affinity chromatography is an effective method for the purification of biological material. A mathematical model has been set up by the material balance in the chromatographic column and the approximate solution has been obtained by the Gaussian expansion equation which expands in the derivatives of normal probability density function. The coefficients of the derivatives are functions of moments which are obtained from the Laplace transformation of the differential equations. Affinity chromatography is affected by changing of pH. Ionization model has been developed to explain the phenomena of pH effect on the affinity chromatography. The relation between the mathematical model and the ionization model is shown.