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제조용 재순환 크로마토그래피에 의한 가시오갈피에 포함된 Acanthoside-D의 분리
Separation of Acanthoside-D in Acanthopanax Senticosus by Preparative Recycle Chromatograpy
인하대학교 초정밀생물분리기술, 인천 402-751 1인하대학교 공과대학 화학공학과, 인천 402-751
Center for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea 1Dept. of Chem. Eng., Inha University, Incheon 402-751, Korea
rowkho@inha.ac.kr
HWAHAK KONGHAK, August 2002, 40(4), 488-491(4), NONE
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Abstract
가시오갈피에 포함되어있는 Acanthoside-D를 추출 및 분리하기 위해서 분석용과 제조용 재순환 크로마토그래피에서의 최적조업조건을 실험적으로 구하였다. 가시오갈피 분말을 에탄올으로 추출하고 헥산으로 분배하였다. 분석용 크로마토그래피에서의 이동상 조건과 주입부피는 각기 water/acetonitrile/methanol=80/14/6 vol%, 20 μl이었고 제조용 재순환 크로마토그래피에서는 각기 70/15/15 vol%, 2 ml이었다. 4번의 재순환을 하여 93%의 순도를 가진 Acanthoside-D를 분취하였다.
To extract and separate Acanthoside-D contained in Acanthopanax Senticosus as a ginseng-like substance, the optimum operating conditions were experimentally determined in the analytical and preparative chromatography. Acanthoside-D was extracted from the powder of the trunk of Acanthopanax Senticosus by enthanol, and the resulting solution was partitioned with n-hexane. The mobile phase composition and injection volume by analytical chromatography were water/acetonitrile/methanol=80/14/6 vol% and 20 μl, respectively while those by recycle chromatography were 70/15/15 vol% and 2 ml. By recycling the sample 4 times, Acanthoside-D with 93% purity was collected.
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Zhao WM, Qin GW, Xu RS, Li XY, Liu JS, Wang Y, Freg M, Fitoterapia, 70, 529 (1999)
Chang SY, Yook CS, Nohara T, Phytochemistry, 50, 1369 (1999)
Hahn DR, Kim CJ, Kim JH, Yakhak Hoeji, 29, 357 (1985)
Nishiyama N, Kahiko T, Iwai A, Saito H, Sanada S, Ida Y, Shoji J, Sho. Zas., 39 (1985)
Brekhman II, Dardymov IV, Lioydia, 32 (1969)
Lee KJ, Kang JH, Row KH, Korean J. Biotechnol. Bioeng., 16, 71 (2001)
"Polymer Analysis by Recycle Prep-LC," JAI Korea Co. Ltd., 4 (2002)
"Recycling Preparative HPLC," JAI Korea Co., Ltd., 1 (2002)
Lee KJ, Kim MH, Row KH, Theor. Appl. Chem. Eng., 6, 3281 (2000)
Shin KH, "The Report of National Institute of Health," 572 (1994)
"High Purity Solvent Guide," Burick and Jackson Laboratories, Inc.: 133 (1984)
