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초임계유체를 이용한 가시오가피로부터 Acanthoside-D의 추출

Extraction of Acanthoside-D in Acanthopanax Senticosus by Supercitical Fluid

송명석 이윤우¹ 김재덕¹ 노경호^†

Myong-Seok Song Yoon-Woo Lee¹ Jae-Duck Kim¹ Kyung Ho Row^†

인하대학교 화학공학과, 초정밀생물분리기술센터, 402-751 인천시 남구 용현동 53 ¹한국과학기술연구원 국가지정 초임계유체연구실, 136-791 서울시 성북구 하월곡동 39-1

Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University, 53, Yonghyeon-dong, Nam-gu, Incheon 402-751, Korea ¹National Research Lab. for Supercritical Fluid, Korea Institute of Science and Technology, 39-1, Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Korea

rowkho@inha.ac.kr

HWAHAK KONGHAK, April 2003, 41(2), 207-212(6), NONE

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Abstract

가시오가피에서 유용성분인 Acanthoside-D를 분리하기 위해서 초임계 이산화탄소와 첨가제로서 물을 사용하여 추출하고 역상 고성능 액체 크로마토그래피(RP-HPLC)를 사용하여 분석하였다. 초임계 이산화탄소의 압력과 온도, 물의 양에 따른 Acanthoside-D의 수율을 Lichrospher 100RP-18(15 μm)의 고정상과 일정용매 조성의 분석조건에서 실험적으로 고찰하였다. 가시오가피에서 Acanthoside-D의 추출은 첨가제를 포함하지 않은 경우에서는 압력이 높을수록 더 높은 수율을 얻었다. 초임계 추출에서의 잔류물에 포함된 Acanthoside-D의 수율은 추출물의 수율 보다 높게 나타났다. 용매추출과 open tubular chromatography(OTC)에 의해서 초임계 추출물을 정제하고 RP-HPLC에 의해서 분리할 수 있었다.

Acanthoside-D from Acanthopanax Senticosus was extracted using carbon dioxide at supercritical state and the additive of water, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The chromatographic column was packed with Lichrospher 100RP-18 (15 μm) and the analysis was performed on the isocratic condition. The yield of Acanthoside-D in supercritical carbon dioxide with pressures, temperatures, and amounts of additive was investigated. In the experimental run without water additives, the yield of Acanthoside-D was increased with higher pressures. But the residual phase of supercritical extraction contained the larger amount of Acanthoside-D at the water additives than no additives. The sample was purified by solvent extraction and open tubular chromatography, and further isolated by RP-HPLC.

Keywords

Acanthopanax Senticosus Acanthoside-D Supercritical Fluid Extraction Additives Residual Phase

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