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Received January 24, 2008
Accepted March 10, 2008
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혈액대용물질의 제조 및 특성 평가
Estimate of Characteristics and Manufacture of Blood Substitutes
Gi-Beum Kim
Jai-Koan Park1
Seong-Jong Kim2
Jong-Soo Kim2
Il-Soo You3
Min-Ho Kim4
Chul-Un Hong5
Jin-Shang Kim
Hyung-Sub Kang†
전북대학교 수의과대학 약리학교실, 561-756 전북 전주시 덕진구 덕진동1가 664-14 1CIC, 565-854 전북 완주군 이서면 이문리 711 2전북대학교 공과대학 화학공학부, 561-756 전북 전주시 덕진구 덕진동1가 664-14 3전북대학교 공과대학 고분자나노공학과, 561-756 전북 전주시 덕진구 덕진동1가 664-14 4전북대학교 의학전문대학원 흉부외과학교실, 561-756 전북 전주시 덕진구 덕진동1가 664-14 5전북대학교 공과대학 바이오메디칼공학부, 561-756 전북 전주시 덕진구 덕진동1가 664-14
Department of Pharmacology, Collage of Veterinary Medicine, Chonbuk National University, 664-14, Duckjin-dong 1ga, Duckjin-gu, Jeonju, Jeonbuk 561-756, Korea 1CIC, 711 Imun-ri Iseo-myeon, Wanju-gun, Jeollabuk-Do, 565-854, Korea 2Division of Chemical Engineering, College of Engineering, Chonbuk National University, 64-14, Duckjin-dong 1ga, Duckjin-gu, Jeonju, Jeonbuk 561-756, Korea 3Department of Polymer Nano Science & Technology, College of Engineering, Chonbuk National University, 64-14, Duckjin-dong 1ga, Duckjin-gu, Jeonju, Jeonbuk 561-756, Korea 4Department of Thoracic and Cardiovascular Surgery, Chonbuk National University Medical Schools, 664-14, Duckjin-dong 1ga, Duckjin-gu, Jeonju, Jeonbuk 561-756, Korea 5Division of Biomedical Engineering, College of Engineering, Chonbuk National University, 664-14, Duckjin-dong 1ga, Duckjin-gu, Jeonju, Jeonbuk 561-756, Korea
kang-hs@chonbuk.ac.kr
Korean Chemical Engineering Research, June 2008, 46(3), 626-632(7), NONE Epub 7 July 2008
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Abstract
본 연구는 헤모글로빈을 계란의 인지질로 microencapsulation한 hemosome과 PFC 유화액을 제조하여 산소전달 특성을 향상시키고자 하였다. 실험결과 fluosol-DA를 sonication하여 평균입자크기가 437 nm의 안정성 있는 유화액을 제조할 수 있었으며, glycerol의 농도를 증가시킴으로써 유화시간을 줄이고 안정도를 높일 수 있었다. 소 혈액으로부터 추출한 헤모글로빈을 달걀노른자로부터 추출한 인지질로 microencapsulation하여 직경 0.8 μm의 hemosome을 얻을 수 있었다. Hemosome의 산소포화도 곡선은 정상혈액의 적혈구와 같이 S자형을 보였으며 P50은 24 mmHg이었다. Hemosome과 혈액을 1:4(V/V%)의 혼합한 혼합용액에서의 산소 포화도는 정상혈액과 유사한 결과를 보였으며, PFC 유화액과 혈액을 1:4(V/V%)로 혼합한 혼합용액에도 정상혈액과 유사한 결과를 보였다.
The purpose of this study was to enhance gas exchange by producing hemosome, a hemoglobin microencapsulated with phospholipid of egg, and perfluorocarbone(PFC) emulsion solution. In the experiment, stable emulsion solution with 437 nm of mean particle size could be produced by Flusol-DA sonication, and shortening of emulsion time could be attained with higher stability as well. 0.8 μm sized hemosome could be produced by microencapsulation of hemoglobin with phospholipid extracted from egg yolk. The pattern of oxygen saturation curve of hemosome was S shape, which is similar to that found in normal blood, and P50 was measured to be 24 mmHg. The oxygen saturation in the mixed solution of hemosome and blood in 1:4(V/V%) ratio was similar to that of normal blood, and the same result was found in the mixed solution of PFC emulsion and blood in 1:4(V/V%) ratio.
Keywords
References
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Kim GB, Kwon TK, Lee SC, Kim SJ, Cheong IS, Oh IH, Kim KJ, Byun YS, Jheong GR, Korean Chem. Eng. Res., 42(2), 151 (2004)
Kim GB, Kim SJ, Hong CU, Kwon TK, Kim NG, Korean J. Chem. Eng., 22(4), 521 (2005)
Kim GB, Hong CU, Kwon TK, J. Artif. Organs, 9, 34 (2006)
Kim GB, Hong CU, Kwon TK, Jpn. J. Appl. Phys., 45(4B), 3811 (2006)
Winslow RM, The Johns Hopkins University Press, Baltimore, USA (1992)
Alpha Therpeutic Corporation. Fluosol 20% intravascular perflorochemical emulsion delivers oxygen to protect the heart during PTCA. Product Monograph, Alpha Therapeutic Corp., Los Angeles (1990)
Ivanitsky GR, Vorobyev SI, Product Monograph; Perftoran Co., Pushchino, Russia (1997)
Park JK, Ph.D. Dissertation, Chonbuk National University (2003)
Szebeni J, Di Iorio EE, Hauser H, Winterhalter KH, Biochemistry, 24, 2827 (1985)
Szoka F, Papahadjopoulos D, Proc. Natl. Acad. Sci., 75, 4194 (1978)
Fujita T, Sumaya T, Yokohama K, Europ. Surg. Res., 3, 436 (1971)
